Neuropilin 1 Monoclonal antibody

Neuropilin 1 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit and More (2)

Applications

WB, IP, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2H3F6

Cat no : 60067-1-Ig

Synonyms

BDCA4, CD304, neuropilin 1, NRP, NRP1, VEGF165R



Tested Applications

Positive WB detected inhuman placenta tissue, fetal human brain tissue, human plasma, 37°C incubated human placenta tissue, pig brain tissue, HeLa cells, rabbit heart tissue, MDA-MB-231 cells, A549 cells, pig heart tissue
Positive IF detected inSH-SY5Y cells, human embronic stem cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60067-1-Ig targets Neuropilin 1 in WB, IP, IF, FC, CoIP, ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity human, pig, rabbit
Cited Reactivityhuman, mouse, Xenopus
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Neuropilin 1 fusion protein Ag0931
Full Name neuropilin 1
Calculated molecular weight 103 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC007737
Gene symbol NRP1
Gene ID (NCBI) 8829
RRIDAB_2150840
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

Protocols

Product Specific Protocols
WB protocol for Neuropilin 1 antibody 60067-1-IgDownload protocol
IF protocol for Neuropilin 1 antibody 60067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
XenopusIF

Elife

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Authors - Max Koppers
humanIF

Acta Biomater

Semaphorin-3A, Neuropilin-1 and Plexin-A1 in Prosthetic-Particle Induced Bone Loss.

Authors - S Saad
human

J Pathol

Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma.

Authors - Eric Morin
humanIF

Am J Cancer Res

CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1.

Authors - Yang Zheng
mouseWB

Pharm Res

Dual Receptor Targeting Cell Penetrating Peptide Modified Liposome for Glioma and Breast Cancer Postoperative Recurrence Therapy.

Authors - Yue Qiu
mouseIF

Exp Eye Res

Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury.

Authors - Victor H Guaiquil