|Positive WB detected in||human placenta tissue, human plasma, fetal human brain tissue, pig brain tissue|
|Positive IF detected in||SH-SY5Y cells, human embronic stem cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60067-1-Ig targets Neuropilin 1 in WB, IF, FC, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Neuropilin 1 fusion protein Ag0931|
|Full Name||neuropilin 1|
|Calculated molecular weight||103 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC007737|
|Gene ID (NCBI)||8829|
|Purification Method||Caprylic acid/ammonium sulfate precipitation|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Neuropilin-1 (NRP1) is a a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a.
Am J Med Genet A
NRP1 haploinsufficiency predisposes to the development of Tetralogy of Fallot.
Cell Death Dis
Cdc42 subcellular relocation in response to VEGF/NRP1 engagement is associated with the poor prognosis of colorectal cancer.
Mol Cell Biochem
miR-338 suppresses the growth and metastasis of OSCC cells by targeting NRP1.
Am J Cancer Res
CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1.
Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma.