Tested Applications
| Positive WB detected in | HEK293 cells, HeLa cells, ROS1728 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66359-1-Ig targets NUP155 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3638 Product name: Recombinant human NUP155 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1043-1391 aa of BC039257 Sequence: LIQVDLADKLLQVASPFLEPHLVRMAKVDQNRVRYMDLLWRYYEKNRSFSNAARVLSRLADMHSTEISLQQRLEYIARAILSAKSSTAISSIAADGEFLHELEEKMEVARIQLQIQETLQRQYSHHSSVQDAVSQLDSELMDITKLYGEFADPFKLAECKLAIIHCAGYSDPILVQTLWQDIIEKELSDSVTLSSSDRMHALSLKIVLLGKIYAGTPRFFPLDFIVQFLEQQVCTLNWDVGFVIQTMNEIGVPLPRLLEVYDQLFKSRDPFWNRMKKPLHLLDCIHVLLIRYVENPSQVLNCERRRFTNLCLDAVCGYLVELQSMSSSVAVQAITGNFKSLQAKLERLH Predict reactive species |
| Full Name | nucleoporin 155kDa |
| Calculated Molecular Weight | 1391 aa, 155 kDa |
| Observed Molecular Weight | 140-155 kDa |
| GenBank Accession Number | BC039257 |
| Gene Symbol | NUP155 |
| Gene ID (NCBI) | 9631 |
| RRID | AB_2881738 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75694 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NUP155 is a nucleoporin, the major component of the nuclear pore complex (NPC) that is essential for nuclear envelope biogenesis. Mutation of NUP155 has been linked to cardiovascular disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NUP155 antibody 66359-1-Ig | Download protocol |
| WB protocol for NUP155 antibody 66359-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NUP155 Monoclonal Antibody (1A7F2), catalog number 66359-1-Ig, has 6 total citations. These appear in Nature Communications, Neuron, Journal of Ethnopharmacology, Frontiers in Pharmacology, BMC Cancer, and Archives of Biochemistry and Biophysics. Research fields span tissue regeneration and alternative polyadenylation, oligodendrocyte differentiation and myelination, heart failure and metabolic remodeling, non-small cell lung cancer progression, breast cancer cell proliferation and apoptosis, and allergic rhinitis involving Th2 cytokine secretion.
| Species | Application | Title |
|---|---|---|
Nat Commun Modulation of Nudt21 levels reveals dose-dependent roles of alternative polyadenylation in tissue regeneration. | ||
Neuron Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. | ||
J Ethnopharmacol Xinfuli granule alleviates metabolic remodeling through inhibition of endoplasmic reticulum stress and mitochondrial injury in heart failure | ||
Front Pharmacol NUP155 and NDC1 interaction in NSCLC: a promising target for tumor progression | ||
BMC Cancer Pan-cancer analysis of NUP155 and validation of its role in breast cancer cell proliferation, migration, and apoptosis
| ||
Arch Biochem Biophys MALAT1 elevates the secretion of Th2 cytokines via miR-422a/NUP155 axis in allergic rhinitis mice |
