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NUP54 Polyclonal antibody, PBS Only (16232-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 16232-1-AP (same clone as 16232-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 16232-1-AP (same clone as 16232-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 16232-1-AP (same clone as 16232-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 16232-1-AP (same clone as 16232-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 16232-1-AP (same clone as 16232-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 16232-1-AP (same clone as 16232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16232-1-AP (NUP54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

NUP54 Polyclonal antibody, PBS Only

Name: NUP54 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16232-1-PBS

NUP54 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16232-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16232-1-PBS targets NUP54 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8882 Product name: Recombinant human NUP54 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC012559 Sequence: MAFNFGAPSGTSGTAAATAAPAGGFGGFGTTSTTAGSAFSFSAPTNTGTTGLFGGTQNKGFGFGTGFGTTTGTSTGLGTGLGTGLGFGGFNTQQQQQTTLGGLFSQPTQAPTQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGYFNNNIPPVEFTQENPFCRFKAVGYSCMPSNKDEDGLVVLVFNKKETEIRSQQQQLVESLHKVLGGNQTLTVNVEGTKTLPDDQTEVVIYVVERSPNGTSRRVPATTLYAHFEQANIKTQLQQLGVTLSMTRTELSPAQIKQLLQNPPAGVDPIIWEQAKVDNPDSEKLIPVPMVGFKELLRRLKVQDQMTKQHQTRL Predict reactive species
Full Name	nucleoporin 54kDa
Calculated Molecular Weight	507 aa, 55 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC012559
Gene Symbol	NUP54
Gene ID (NCBI)	53371
RRID	AB_10644484
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z3B4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.