CoraLite® Plus 488-conjugated NXN Monoclonal antibody

NXN Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C6A4

Cat no : CL488-66603

Synonyms

FLJ12614, NRX, nucleoredoxin, NXN, TRG 4

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-66603

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 NXN antibody (CL488-66603, Clone: 2C6A4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-66603

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NXN (CL488-66603, Clone:2C6A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL488-66603 targets NXN in IF, FC (Intra) applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NXN fusion protein Ag9248
• Full Name: nucleoredoxin
• Calculated molecular weight: 435 aa, 48 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: BC009327
• Gene symbol: NXN
• Gene ID (NCBI): 64359
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.