CoraLite® Plus 488-conjugated NXN Monoclonal antibody
NXN Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2C6A4
Cat no : CL488-66603
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HEK-293 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66603 targets NXN in IF, FC (Intra) applications and shows reactivity with Human, Mouse , Rat samples.
Tested Reactivity | Human, Mouse , Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | NXN fusion protein Ag9248 |
Full Name | nucleoredoxin |
Calculated molecular weight | 435 aa, 48 kDa |
Observed molecular weight | 48 kDa |
GenBank accession number | BC009327 |
Gene symbol | NXN |
Gene ID (NCBI) | 64359 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 NXN antibody CL488-66603 | Download protocol |
FC protocol for CL Plus 488 NXN antibody CL488-66603 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |