ChromoTek Nano-CaptureLigand® anti-human Ig kappa (light chain) recombinant VHH, biotinylated [CTK0125]

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• Nano-CaptureLigand® anti-human Ig kappa (light chain) recombinant VHH, biotinylated [CTK0125] (MG-2231B)

Validation Data Gallery

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  Capture of Fab fragments (Biolayer Interferometry)
  The Nano-CaptureLigand™ human Ig, kappa LC-specific VHH, biotinylated, was immobilized on Streptavidin biosensors and used to capture Fab fragments (κ light chain) of therapeutic human IgGs . Association for 600 s followed by dissociation for 600 s.

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  Kinetic analysis of the Adalimumab Fab fragment via Biolayer Interferometry
  The Nano-CaptureLigand® human Ig, kappa LC-specific VHH, biotinylated, was immobilized on Streptavidin biosensors and used to capture the Fab fragment of the therapeutic IgG Adalimumab. The Adalimumab Fab fragment was then characterized for its binding kinetics by the titration of its target protein TNFα (HumanKine HZ-1014; 7 to 62 nM;a ssociation for 600 s followed by dissociation for 600 s). The resulting dissociation constant KD of 0.7 nM is consistent with literature values.

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  Capture of therapeutic IgG (Biolayer Interferometry)
  The Nano-CaptureLigand™ human Ig, kappa LC-specific VHH, biotinylated, was immobilized on Streptavidin biosensors and used to capture therapeutic human IgGs (100 nM each) of varying formats (see below). All human IgGs with a κ light chain were successfully captured, while IgG λ was not. Association for 300 s followed by dissociation for 300 s.

The Nano-CaptureLigand® human Ig, kappa-LC captures and immobilizes human Ig kappa light chain (LC) antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated VHH (Nanobody) that specifically binds to human Ig kappa LC antibodies with high affinity and in a site-directed manner.

Cat No. MG-2231B

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host/Type

Alpaca VHH

Reactivity

Human Ig, kappa light chain;no cross-reactivity to human Ig lambda light chain

Synonyms

biotinylated secondary VHH

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Product Information

The Nano-CaptureLigand® human Ig, kappa-LC captures and immobilizes human Ig kappa light chain (LC) antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated VHH (Nanobody) that specifically binds to human Ig kappa LC antibodies with high affinity and in a site-directed manner.

• Description: The next level of antibody immobilization to BLI and SPR biosensors;

  • Site-directed and specific immobilization of the Fab-fragment of human Ig through the lambda light chain to streptavidin coated biosensors without biotinylation of the antibody;

  • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems);

  • High affinity for stable baseline without significant dissociation;

  • >10x regeneration for multiple reuse;

  • High specificity enables human Ig lambda antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

• Applications: BLI, SPR, ELISA
• Host: Alpaca
• Reactivity: Human Ig, kappa light chain,; no cross-reactivity to: human Ig lambda light chain
• Conjugate: Biotin
• Clone#: CTK0125
• Class: Recombinant
• MW: 14.7 kDa
• Type: Nanobody
• Immunogen: Human Ig Kappa Light Chain
• Form: Liquid
• RRID: /
• Storage buffer: 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, 0.09 % sodium azide
• Storage condition: -20°C
• Shipping: Blue ice

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