Validation Data Gallery
![<b>Capture of therapeutic IgG (Biolayer Interferometry)
</b> <br>Nano-CaptureLigand®, anti-human IgG (Fc) recombinant VHH, biotinylated [clone CTK0124], was immobilized on Streptavidin biosensors and used to capture therapeutic human IgGs (100 nM each) of varying formats (see below). All formats were successfully captured with very low dissociation observed. Association for 300 s followed by dissociation for 300 s.](https://www.ptglab.com/products/pictures/Result-MG-2228B.jpg "<b>Capture of therapeutic IgG (Biolayer Interferometry)
</b> <br>Nano-CaptureLigand®, anti-human IgG (Fc) recombinant VHH, biotinylated [clone CTK0124], was immobilized on Streptavidin biosensors and used to capture therapeutic human IgGs (100 nM each) of varying formats (see below). All formats were successfully captured with very low dissociation observed. Association for 300 s followed by dissociation for 300 s.")
![<b>Capture of human IgG subtypes (Biolayer Interferometry)</b>
<br>The Nano-CaptureLigand®, anti-human IgG (Fc) recombinant VHH, biotinylated [CTK0124], was immobilized on Streptavidin biosensors and used to capture human IgG1, IgG2, IgG3, or IGg4 (titrated from 0.8 to 50 nM). Association for 600 s followed by dissociation for 600 s. All subtypes of human IgG are captured with very high affinity.](https://www.ptglab.com/products/pictures/Result-MG-2228B_20261008135233410.jpg "<b>Capture of human IgG subtypes (Biolayer Interferometry)</b>
<br>The Nano-CaptureLigand®, anti-human IgG (Fc) recombinant VHH, biotinylated [CTK0124], was immobilized on Streptavidin biosensors and used to capture human IgG1, IgG2, IgG3, or IGg4 (titrated from 0.8 to 50 nM). Association for 600 s followed by dissociation for 600 s. All subtypes of human IgG are captured with very high affinity.")
Product Information
The Nano-CaptureLigand® human IgG (Fc) captures and immobilizes human IgG to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated VHH (Nanobody) that specifically binds to human IgG Fc fragments with high affinity and in a site-directed manner.
This clone is not cross-reactive to rabbit IgG.
This clone is cross-reactive to cynomolgus IgG.
| Description | The next level of antibody immobilization to BLI and SPR biosensors • Site-directed and specific immobilization of the Fab-fragment of human Ig through the lambda light chain to streptavidin coated biosensors without biotinylation of the antibody • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems) • High affinity for stable baseline without significant dissociation • >10x regeneration for multiple reuse • High specificity enables human Ig lambda antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples |
| Host | Alpaca |
| Reactivity | Human IgG (Fc), no cross-reactivity to: mouse IgG, goat IgG, rabbit IgG, rat IgG, cross-reactive to: cyno |
| Conjugate | Biotin |
| Clone# | CTK0124 |
| Class | Recombinant |
| MW | 14.3 kDa |
| Type | Nanobody |
| Immunogen | 畈慭杉⁇捆 |
| Form | Liquid |
| RRID | / |
| Storage buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide |
| Storage condition | -20°C |
| Shipping | Blue ice |