ChromoTek Nano-Secondary® anti-human IgG / anti-rabbit IgG recombinant VHH, Alkaline Phosphatase

Nano-Secondary ® anti rabbit IgG / anti human IgG recombinant VHH, Alkaline Phosphatase is an anti-human IgG and anti-rabbit IgG secondary Nanobody (VHH). It is a fusion of a VHH that binds to human IgG and rabbit IgG with high affinity & specificity, and one AP molecule. Note that AP will dimerize. This product uses recombinant, animal-production free Alkaline-Phosphatase, expressed as fusion-protein with the VHH in E.Coli.
Cat No. srbhuAP

Applications

ELISA

Reactivity

Rabbit IgG / Human IgG

Host/Type

Alpaca

Conjugate

Alkaline-Phosphatase (recombinantly produced)

Nano-Secondary reagent, Secondary VHH, AP, Alkaline Phosphatase

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Product Information

Nano-Secondary ® anti rabbit IgG / anti human IgG recombinant VHH, Alkaline Phosphatase is an anti-human IgG and anti-rabbit IgG secondary Nanobody (VHH). It is a fusion of a VHH that binds to human IgG and rabbit IgG with high affinity & specificity, and one AP molecule. Note that AP will dimerize. This product uses recombinant, animal-production free Alkaline-Phosphatase, expressed as fusion-protein with the VHH in E.Coli.

DescriptionAlkaline Phosphatase allows for a broader range enzymatic detection compared to HRP. The VHH format delivers excellent specificity, with no cross-reactivity against serum proteins from other species.
ApplicationsELISA
Host/TypeAlpaca
ReactivityRabbit IgG / Human IgG
ClassVHH
FormLiquid
ConjugateAlkaline-Phosphatase (recombinantly produced)
Molecular weight61.5 kDa (monomer); 123 kDa (dimer)
ImmunogenPurified rabbit IgG
AffinityKD = 0.2 nM
RRID/
Storage Buffer20 mM Tris pH 8.0, 250 mM NaCl, 1 mM MgCl2, 0.1 mM ZnSO4, 50% glycerol
Storage condition-20°C / -80°C
ShippingBlue ice
Size1ml