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Validation Data Gallery

  • Anti-human IL-6 sandwich ELISA. NeutraKine® anti-human IL-6 Mouse IgG1 McAb (Cat.No. 69001-1-Ig) direct coated 10 µg/mL on a MaxiSorp plate captures HumanKine® recombinant human IL-6 protein (Cat.No. HZ-1019) titrated from 200 – 0,01 nM. Primary anti-human IL-6 polyclonal rabbit antibody (Cat.No. 21865-1-AP) 1:1000 binds to the captured IL-6 antigen and is detected by the Nano-Secondary ® anti-human IgG / anti-rabbit IgG recombinant VHH, Alkaline Phosphatase (Cat.No. srbhuAP) 1:5000.

    Anti-human IL-6 sandwich ELISA. NeutraKine® anti-human IL-6 Mouse IgG1 McAb (Cat.No. 69001-1-Ig) direct coated 10 µg/mL on a MaxiSorp plate captures HumanKine® recombinant human IL-6 protein (Cat.No. HZ-1019) titrated from 200 – 0,01 nM. Primary anti-human IL-6 polyclonal rabbit antibody (Cat.No. 21865-1-AP) 1:1000 binds to the captured IL-6 antigen and is detected by the Nano-Secondary ® anti-human IgG / anti-rabbit IgG recombinant VHH, Alkaline Phosphatase (Cat.No. srbhuAP) 1:5000.

ChromoTek Nano-Secondary® anti-human IgG/anti-rabbit IgG recombinant VHH, Alkaline Phosphatase

Nano-Secondary ® anti rabbit IgG / anti human IgG recombinant VHH, Alkaline Phosphatase is an anti-human IgG and anti-rabbit IgG secondary Nanobody (VHH). It is a fusion of a VHH that binds to human IgG and rabbit IgG with high affinity & specificity, and one AP molecule. Note that AP will dimerize. This product uses recombinant, animal-production free Alkaline-Phosphatase, expressed as fusion-protein with the VHH in E.Coli.
Cat No. srbhuAP

Review Product

Applications

ELISA

Reactivity

Rabbit IgG / Human IgG

Host/Type

Alpaca

Conjugate

Alkaline-Phosphatase (recombinantly produced)

Synonyms

Nano-Secondary reagent, Secondary VHH, AP, Alkaline Phosphatase

Formats:  Alkaline Phosphatase
SKU/Size: 

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Freight/Packing: -

Quantity

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Product Information

Nano-Secondary ® anti rabbit IgG / anti human IgG recombinant VHH, Alkaline Phosphatase is an anti-human IgG and anti-rabbit IgG secondary Nanobody (VHH). It is a fusion of a VHH that binds to human IgG and rabbit IgG with high affinity & specificity, and one AP molecule. Note that AP will dimerize. This product uses recombinant, animal-production free Alkaline-Phosphatase, expressed as fusion-protein with the VHH in E.Coli.

DescriptionAlkaline Phosphatase allows for a broader range enzymatic detection compared to HRP. The VHH format delivers excellent specificity, with no cross-reactivity against serum proteins from other species.
ApplicationsELISA
Host/TypeAlpaca
ReactivityRabbit IgG / Human IgG
ClassVHH
FormLiquid
ConjugateAlkaline-Phosphatase (recombinantly produced)
Molecular weight61.5 kDa (monomer); 123 kDa (dimer)
ImmunogenPurified rabbit IgG
AffinityKD = 0.2 nM
RRID/
Storage Buffer20 mM Tris pH 8.0, 250 mM NaCl, 1 mM MgCl2, 0.1 mM ZnSO4, 50% glycerol
Storage condition-20°C / -80°C
ShippingBlue ice
Size1ml