NeuN Monoclonal antibody

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  IHC staining of rat cerebellum using 66836-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of rat cerebellum using 66836-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human brain using 66836-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat cerebellum using 66836-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green).

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  IF Staining of mouse cerebellum using 66836-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green).

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  FC experiment of SH-SY5Y using 66836-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NeuN (66836-1-Ig, Clone:3A4C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NeuN Monoclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Cat No. 66836-1-Ig

Clone No.3A4C1

Publications(141)

Reviews (5)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IF-P, FC (Intra), ELISA

Synonyms

Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C

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Tested Applications

• Positive IHC detected in: rat cerebellum tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat cerebellum tissue, mouse cerebellum tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

This antibody is not suitable for staining in frozen section.

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 6 publications below
• IF: See 128 publications below
• FC: See 1 publications below

Product Information

66836-1-Ig targets NeuN in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NeuN fusion protein Ag28016
• Full Name: hexaribonucleotide binding protein 3
• GenBank Accession Number: NM_001082575
• Gene Symbol: NeuN
• Gene ID (NCBI): 146713
• RRID: AB_2882179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

Publications

Species	Application	Title
mouse	IF	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
rat	IF	Microbiome; The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.; Authors - Weiping Xiao
rat	IF	Redox Biol; LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX; Authors - Dongya Jiang
rat	IF	Cell Death Dis; ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis; Authors - Yaxuan Zhang
mouse	IF	Cell Death Dis; Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy; Authors - Yang Deng
mouse	IF	Diabetes; Regulatory Role of NF-κB on HDAC2 and Tau Hyperphosphorylation in Diabetic Encephalopathy and the Therapeutic Potential of Luteolin; Authors - Qian Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carla (Verified Customer) (02-03-2025)

We didn't have any good results

• Applications: Immunofluorescence
• Cell Tissue Type: organoid/Brain tissue

FH

Kenzo (Verified Customer) (01-05-2024)

This monoclonal NeuN worked well for mouse tissues.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Retina

FH

Silvia (Verified Customer) (08-11-2022)

it works well for Immunofluorescence on mature neurons

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: hiPSCs-derived neurons

FH

Delphine (Verified Customer) (07-25-2022)

Immunohistochemistry with the NeuN antibody on frozen spinal cord worked but the labeling must be optimized because there is a lot of background noise.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: tissue

FH

q (Verified Customer) (01-05-2022)

It is OK to use it in WB, but several non-specific bands above the expected MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

NeuN Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66836-1-Ig)