Tested Applications
| Positive WB detected in | HeLa cells, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human liver cancer tissue, human pancreas cancer tissue, rat small intestine tissue, human kidney tissue, rat stomach tissue, mouse testis tissue, human stomach cancer tissue, mouse stomach tissue, human placenta tissue, human thyroid cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66698-1-Ig targets OCIAD1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9977 Product name: Recombinant human OCIAD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC003409 Sequence: MNGRADFREPNAEVPRPIPHIGPDYIPTEEERRVFAECNDESFWFRSVPLAATSMLITQGLISKGILSSHPKYGSIPKLILACIMGYFAGKLSYVKTCQEKFKKLENSPLGEALRSGQARRSSPPGHYYQKSKYDSSVSGQSSFVTSPAADNIEMLPHYEPIPFSSSMNESAPTGITDHIVQGPDPNLEESPKRKNITYEELRNKNRESYEVSLTQKTDPSVRPMHERVPKKEVKVNKYGDTWDE Predict reactive species |
| Full Name | OCIA domain containing 1 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC003409 |
| Gene Symbol | OCIAD1 |
| Gene ID (NCBI) | 54940 |
| RRID | AB_2882051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NX40 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OCIAD1 antibody 66698-1-Ig | Download protocol |
| IHC protocol for OCIAD1 antibody 66698-1-Ig | Download protocol |
| WB protocol for OCIAD1 antibody 66698-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OCIAD1 Monoclonal antibody (1C10C3), catalog number 66698-1-Ig, has 2 total citations. These appear in Clinical Respiratory Journal and eLife. The cited research spans lung adenocarcinoma subtyping via integrated proteomics and single-cell RNA sequencing, and CRISPRi-based identification of OCIAD1 as a regulatory determinant of mitochondrial Complex III assembly in human cells.
| Species | Application | Title |
|---|---|---|
Clin Respir J Integrated Proteomic and scRNA-Seq Analysis Reveals Pyroptosis-Related Subtypes in Lung Adenocarcinoma. | ||
Elife Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
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