OGN Monoclonal antibody

OGN Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5F11

Cat no : 66382-1-Ig

Synonyms

Mimecan, OG, OGN, OIF, osteoglycin, Osteoinductive factor, SLRR3A

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Validation Data Gallery

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  WB analysis of human artery using 66382-1-Ig

  Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (OGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig artery using 66382-1-Ig

  Pig artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (OGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66382-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66382-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 66382-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66382-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human artery tissue, pig artery tissue
• Positive IHC detected in: human liver tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66382-1-Ig targets OGN in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OGN fusion protein Ag3484
• Full Name: osteoglycin
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC037273
• Gene Symbol: OGN
• Gene ID (NCBI): 4969
• RRID: AB_2881758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.