Osteoglycin Monoclonal antibody, PBS Only (Capture)

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  WB analysis of human artery using 66382-1-Ig (same clone as 66382-1-PBS)

  Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  WB analysis of pig artery using 66382-1-Ig (same clone as 66382-1-PBS)

  Pig artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50723-1

  Sandwich ELISA standard curve of MP50723-1, Human Osteoglycin Monoclonal Matched Antibody Pair - PBS only. 66382-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3484. 66382-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP50723-1

  Plasma of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.7 ng/mL with a range of 1.2-2.2 ng/mL.

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  Sample test of MP50723-1

  Serum of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.9 ng/mL with a range of 1.4-2.3 ng/mL.

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  IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66382-1-Ig (same clone as 66382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50723-1

  Cytometric bead array standard curve of MP50723-1, OGN/Osteoglycin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66382-1-PBS. Detection antibody: 66382-2-PBS. Standard:Ag3484. Range: 0.098-100 ng/mL.

Osteoglycin Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 66382-1-PBS

Clone No.2A5F11

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

OGN, Osteoinductive factor, OIF, OG, Mimecan

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Product Information

66382-1-PBS targets Osteoglycin as part of a matched antibody pair:

MP50723-1: 66382-1-PBS capture and 66382-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Osteoglycin fusion protein Ag3484
• Full Name: osteoglycin
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC037273
• Gene Symbol: OGN
• Gene ID (NCBI): 4969
• RRID: AB_2881758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P20774
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.