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OLFM3 Polyclonal antibody, PBS Only (12596-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of PC-3 using 12596-1-AP (same clone as 12596-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 12596-1-AP (same clone as 12596-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 12596-1-AP (same clone as 12596-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
WB analysis of A2780 using 12596-1-AP (same clone as 12596-1-PBS)
A2780 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 12596-1-AP (same clone as 12596-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12596-1-AP (same clone as 12596-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12596-1-AP (same clone as 12596-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12596-1-AP (OLFM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12596-1-AP (same clone as 12596-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OLFM3 antibody (12596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12596-1-PBS in a different storage buffer formulation.

OLFM3 Polyclonal antibody, PBS Only

Name: OLFM3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12596-1-PBS

OLFM3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12596-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Olfactomedin 3, NOE3, Noelin 3, NOELIN3, Noelin-3

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12596-1-PBS targets OLFM3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3207 Product name: Recombinant human OLFM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 159-458 aa of BC022531 Sequence: NLSAVLTGIQEEIGAYDYEELHQRVLSLETRLRDCMKKLTCGKLMKITGPVTVKTSGTRFGAWMTDPLASEKNNRVWYMDSYTNNKIVREYKSIADFVSGAESRTYNLPFKWAGTNHVVYNGSLYFNKYQSNIIIKYSFDMGRVLAQRSLEYAGFHNVYPYTWGGFSDIDLMADEIGLWAVYATNQNAGNIVISQLNQDTLEVMKSWSTGYPKRSAGESFMICGTLYVTNSHLTGAKVYYSYSTKTSTYEYTDIPFHNQYFHISMLDYNARDRALCAWNNGHQVLFNVTLFHIIKTEDDT Predict reactive species
Full Name	olfactomedin 3
Calculated Molecular Weight	458 aa, 53 kDa
Observed Molecular Weight	50-55 kDa
GenBank Accession Number	BC022531
Gene Symbol	OLFM3
Gene ID (NCBI)	118427
RRID	AB_2157228
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96PB7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

OLFM3, also known as Noelin-3, belongs to a larger family of proteins containing the olfactomedin domain. OLFM3, which is most closely related to OLFM2, is expressed in the retinal ganglion and inner nuclear layers of the adult rat retina (PMID: 12019210).