Osteoglycin Polyclonal antibody, PBS Only

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• Osteoglycin Polyclonal antibody, PBS Only (12755-1-PBS)

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  WB analysis of NIH/3T3 using 12755-1-AP (same clone as 12755-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12755-1-AP (same clone as 12755-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 12755-1-AP (same clone as 12755-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12755-1-AP (same clone as 12755-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Osteoglycin antibody (12755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

Osteoglycin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12755-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

12755-1-PBS targets Osteoglycin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3484

  Product name: Recombinant human OGN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-298 aa of BC037273

  Sequence: MKTLQSTLLLLLLVPLIKPAPPTQQDSRIIYDYGTDNFEESIFSQDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSGSVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIEDGTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFICLKRLPIGSYF

• Full Name: osteoglycin
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC037273
• Gene Symbol: OGN
• Gene ID (NCBI): 4969
• RRID: AB_10646479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20774
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.