P15RS Polyclonal antibody

• Featured Product
• KD/KO Validated

P15RS Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 23652-1-AP

Synonyms

RPRD1A, Regulation of nuclear pre-mRNA domain-containing protein 1A, p15INK4B-related protein, p15INK4B related protein, HsT3101

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Validation Data Gallery

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  WB analysis using 23652-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23652-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 23652-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 23652-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 23652-1-AP (P15RS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 23652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 23652-1-AP (P15RS antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 23652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P15RS antibody (23652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 23652-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P15RS antibody (23652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, mouse liver tissue, mouse skeletal muscle tissue, L02 cells, Jurkat cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

23652-1-AP targets P15RS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P15RS fusion protein Ag20409
• Full Name: regulation of nuclear pre-mRNA domain containing 1A
• Calculated Molecular Weight: 312 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC010136
• Gene Symbol: RPRD1A
• Gene ID (NCBI): 55197
• RRID: AB_2879306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15(INK4b) and may have a role in cell cycle regulation. It contains a RAR domain that is involved in regulation of nuclear pre-mRNA, which suggests that P15RS may act as a nuclear regulation protein [PMID:12470661]. It also interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Besides, RPRD1A regulates cell cycle genes and attenuates WNT signaling [PMID: 20739273], and acts as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle [PMID: 22231121].

Publications

Species	Application	Title
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen
human	IP,WB,IHC,IF	Cell Death Dis; RPRD1A stabilizes NRF2 and aggravates HCC progression through competing with p62 for TRIM21 binding.; Authors - Xiaofan Feng ; KD Validated
human	WB	Mol Cell Biol; An Important Role for RPRD1B in the Heat Shock Response; Authors - Simona Cugusi
mouse	WB	Commun Biol; Circ-0075305 hinders gastric cancer stem cells by indirectly disrupting TCF4-β-catenin complex and downregulation of SOX9; Authors - Qi-Yue Chen