P16-INK4A Recombinant antibody

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  WB analysis using 81373-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81373-8-RR (P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81373-8-RR

  Biolayer interferometry (BLl) kinetic assays of 81373-8-RR against Human P16-INK4A were performed. The affinity constant is 0.336 nM.

P16-INK4A {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 81373-8-RR

Clone No.241438C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, TF-1 cells, SiHa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81373-8-RR targets P16-INK4A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: P16-INK4A fusion protein Ag1328
• Full Name: cyclin-dependent kinase inhibitor 2A
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC021998
• Gene Symbol: CDKN2A
• Gene ID (NCBI): 1029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42771
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma. Long-term p16(INK4a) expression pushes cells to enter senescence, an irreversible cell-cycle arrest that precludes the growth of would-be cancer cells but also contributes to cellular aging (PMID: 24136988).