P2RX4 Monoclonal antibody

P2RX4 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1A5A6

Cat no : 66416-1-Ig

Synonyms

ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue, A549 cells, Neuro-2a cells, human brain tissue, pig brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66416-1-Ig targets P2RX4 in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P2RX4 fusion protein Ag4445
Full Name purinergic receptor P2X, ligand-gated ion channel, 4
Calculated molecular weight 388 aa, 43 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC033826
Gene symbol P2RX4
Gene ID (NCBI) 5025
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).

Protocols

Product Specific Protocols
WB protocol for P2RX4 antibody 66416-1-IgDownload protocol
IHC protocol for P2RX4 antibody 66416-1-IgDownload protocol
FC protocol for P2RX4 antibody 66416-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols