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Palladin Polyclonal antibody
Palladin Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 10853-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells|
|Positive IP detected in||HeLa cells, HEK-293 cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human colon tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10853-1-AP targets Palladin in WB, IP, IHC, IF, FC, COIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Palladin fusion protein Ag1288|
|Full Name||palladin, cytoskeletal associated protein|
|Calculated molecular weight||1383 aa, 151 kDa|
|Observed molecular weight||95 kDa, 140 kDa|
|GenBank accession number||BC013867|
|Gene ID (NCBI)||23022|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.
|Product Specific Protocols|
|WB protocol for Palladin antibody 10853-1-AP||Download protocol|
|IHC protocol for Palladin antibody 10853-1-AP||Download protocol|
|IF protocol for Palladin antibody 10853-1-AP||Download protocol|
|IP protocol for Palladin antibody 10853-1-AP||Download protocol|
|FC protocol for Palladin antibody 10853-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Actin cross-linking protein palladin and spermatogenesis.
Biochim Biophys Acta
Differential phosphorylation of Akt1 and Akt2 by protein kinase CK2 may account for isoform specific functions.
TGFβ functionalized starPEG-heparin hydrogels modulate human dermal fibroblast growth and differentiation.
Palladin expression is a conserved characteristic of the desmoplastic tumor microenvironment and contributes to altered gene expression.
Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier.
Am J Physiol Endocrinol Metab
Dynein 1 supports spermatid transport and spermiation during spermatogenesis in the rat testis.