PCK1/2 Polyclonal antibody, PBS Only

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• PCK1/2 Polyclonal antibody, PBS Only (14892-1-PBS)

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  WB analysis of mouse brain using 14892-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14892-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14892-1-AP (same clone as 14892-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14892-1-AP (same clone as 14892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK1/2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 14892-1-AP (same clone as 14892-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PCK1/2 Polyclonal antibody (14892-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

PCK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 14892-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PCK2, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

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Product Information

14892-1-PBS targets PCK1/2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6681

  Product name: Recombinant human PCK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 292-640 aa of BC001454

  Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

• Full Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 66-71 kDa
• GenBank Accession Number: BC001454
• Gene Symbol: PCK2
• Gene ID (NCBI): 5106
• RRID: AB_2160044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16822
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.