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PCNA Monoclonal antibody

PCNA Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

10D10E11

Cat no : 60097-1-Ig

Synonyms

Cyclin, PCNA


"PCNA Antibodies" Comparison

View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
  • IF Staining of mouse heart using 60097-1-Ig

    IF Staining of mouse heart using 60097-1-Ig

    E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.

  • IHC staining of human gliomas using 60097-1-Ig

    IHC staining of human gliomas using 60097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 60097-1-Ig

    IF Staining of HepG2 using 60097-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60097-1-Ig (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HEK-293 using 60097-1-Ig

    WB analysis of HEK-293 using 60097-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60097-1-Ig (PCNA Antibody) at various dilution incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 60097-1-Ig

    WB analysis of HeLa using 60097-1-Ig

    Western blot analysis of PCNA in various cell lines using Proteintech antibody 60097-1-Ig at a dilution of 1:10000 (exposed for 2 seconds) incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 60097-1-Ig

    IP experiment of HepG2 using 60097-1-Ig

    IP Result of anti-PCNA (IP:60097-1-Ig, 4ug; Detection:60097-1-Ig 1:300) with HepG2 cells lysate 3000ug.

  • FC experiment of MCF-7 using 60097-1-Ig

    FC experiment of MCF-7 using 60097-1-Ig

    1X10^6 MCF-7 cells were stained with 0.2ug PCNA antibody (60097-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

  • WB analysis of HEK-293 using 60097-1-Ig

    WB analysis of HEK-293 using 60097-1-Ig

    WB result of PCNA antibody (60097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCNA transfected HEK-293 cells.


Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman gliomas tissue, human tonsillitis tissue, human breast cancer tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse heart tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

60097-1-Ig targets PCNA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCNA fusion protein Ag7416
Full Name proliferating cell nuclear antigen
Calculated molecular weight 29 kDa/31 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000491
Gene symbol PCNA
Gene ID (NCBI) 5111
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Protocols

Product Specific Protocols
WB protocol for PCNA antibody 60097-1-IgDownload protocol
IHC protocol for PCNA antibody 60097-1-IgDownload protocol
IF protocol for PCNA antibody 60097-1-IgDownload protocol
IP protocol for PCNA antibody 60097-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Neurochem Res

Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier.

Authors - Liu Xiaoying
humanWB

J Cell Physiol

HOXC13-AS promotes breast cancer cell growth through regulating miR-497-5p/PTEN axis.

Authors - Xiaowei Li
rabbitWB

Int J Mol Med

Bradykinin potentially stimulates cell proliferation in rabbit corneal endothelial cells through the ZO‑1/ZONAB pathway.

Authors - Lixian He
humanIHC

Biochim Biophys Acta

BMP-6 inhibits cell proliferation by targeting microRNA-192 in breast cancer.

Authors - Hu Fen F
mouseIHC

Mol Ther Oncolytics

A Novel Antitumor Strategy: Simultaneously Inhibiting Angiogenesis and Complement by Targeting VEGFA/PIGF and C3b/C4b.

Authors - Huiling Wang
ratWB

Biomed Res Int

Effect of Quercetin on PC12 Alzheimer's Disease Cell Model Induced by Aβ25-35 and Its Mechanism Based on Sirtuin1/Nrf2/HO-1 Pathway.

Authors - Xinjun Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (08-07-2021)

PCNA in human primary fibroblasts in culture. Passage number 15-25. 15 ug of protein. 4-15% TGX gel. Blocking BSA 3% in PBST. Primary ab 1:1000 in BSA O/N 4ºC. Secondary ab 1:5000 HRP.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human primary fibroblasts
PCNA Antibody Western Blot validation (1:1000 dilution) in human primary fibroblasts (Cat no:60097-1-Ig)