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PD-L2/CD273 Polyclonal antibody, PBS Only (27406-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 27406-1-AP (same clone as 27406-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 27406-1-AP (same clone as 27406-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 27406-1-AP (same clone as 27406-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 27406-1-AP (same clone as 27406-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PDCD1LG2/PD-L2 antibody (27406-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

PD-L2/CD273 Polyclonal antibody, PBS Only

Name: PD-L2/CD273 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 27406-1-PBS

PD-L2/CD273 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 27406-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, B7 DC, B7DC

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

27406-1-PBS targets PD-L2/CD273 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26384 Product name: Recombinant human PDCD1LG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-220 aa of BC074766 Sequence: LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPT Predict reactive species
Full Name	programmed cell death 1 ligand 2
Calculated Molecular Weight	273 aa, 31 kDa
Observed Molecular Weight	45 kDa
GenBank Accession Number	BC074766
Gene Symbol	PD-L2
Gene ID (NCBI)	80380
RRID	AB_3085954
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BQ51
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).