PDLIM3 Polyclonal antibody, PBS Only

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• PDLIM3 Polyclonal antibody, PBS Only (13199-2-PBS)

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  WB analysis of human heart using 13199-2-AP (same clone as 13199-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 13199-2-AP (same clone as 13199-2-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13199-2-AP (same clone as 13199-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13199-2-AP (PDLIM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

PDLIM3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 13199-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ALP, Actinin associated LIM protein, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein, PDZ and LIM domain 3

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Product Information

13199-2-PBS targets PDLIM3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3934

  Product name: Recombinant human PDLIM3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC027870

  Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQNEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA

• Full Name: PDZ and LIM domain 3
• Calculated Molecular Weight: 316 aa, 34 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC027870
• Gene Symbol: PDLIM3
• Gene ID (NCBI): 27295
• RRID: AB_2161653
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53GG5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDLIM3, also known as ALP, contains a PDZ domain and a LIM domain. PDLIM3 binds the spectrin-like repeats of alpha-actinin-2 and colocalizes with alpha-actinin-2 at the Z lines of skeletal muscle.