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PDS5A Monoclonal antibody

PDS5A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67520-1-Ig
Clone No.1C8D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

SCC 112, PIG54, PDS5, KIAA0648, Cell proliferation-inducing gene 54 protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67520-1-Ig targets PDS5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDS5A fusion protein Ag11525 Predict reactive species
Full Name PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
Calculated Molecular Weight 1297 aa, 147 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC009650
Gene Symbol PDS5A
Gene ID (NCBI) 23244
RRIDAB_2882740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ29RF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDS5A antibody 67520-1-IgDownload protocol
IHC protocol for PDS5A antibody 67520-1-IgDownload protocol
IF protocol for PDS5A antibody 67520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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