CD31 Monoclonal antibody

CD31 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A1E2

Cat no : 66065-1-Ig

Synonyms



Tested Applications

Positive WB detected inJurkat cells, THP-1 cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66065-1-Ig is CD31 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC022512
Gene symbol PECAM1
Gene ID (NCBI) 5175
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 66065-1-IgDownload protocol
IHC protocol for CD31 antibody 66065-1-IgDownload protocol
IF protocol for CD31 antibody 66065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

Construction of a human cell landscape at single-cell level.

Authors - Xiaoping Han

Biomaterials

An iRGD-conjugated prodrug micelle with blood-brain-barrier penetrability for anti-glioma therapy.

Authors - Lu Lu
humanIF

Small

Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle.

Authors - Haiming Zhao
humanIF

Theranostics

Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.

Authors - Yumin Li
mouseIF

Theranostics

Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.

Authors - Ke Feng
mouseIHC

Cancer Lett

CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

Authors - Nils Ludwig