CD31 Antibody

CD31 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A1E2

Cat no : 66065-1-Ig

Synonyms

CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker



Tested Applications

Positive WB detected inJurkat cells, THP-1 cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 66065-1-Ig immunogen is Fusion Protein CD31 protein expressed in E. coli.

Reactivity human
Cited Species human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
Observed molecular weight 120-130 kDa
GenBank accession number BC022512
Gene symbol PECAM1
Gene ID (NCBI) 5175
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 66065-1-IgDownload protocol
IHC protocol for CD31 antibody 66065-1-IgDownload protocol
IF protocol for CD31 antibody 66065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Immunother Cancer

Tertiary lymphoid organs in the inflammatory myopathy associated with PD-1 inhibitors.

Authors - Shiro Matsubara

Anticancer Res

Expression of CD31 in Mycosis Fungoides.

Authors - Alina Jankowska-Konsur
humanWB

Int J Mol Med

CCN1/Cyr61 enhances the function of hepatic stellate cells in promoting the progression of hepatocellular carcinoma.

Authors - Zhi-Qiang Li

Oncol Rep

Downregulation of tetrahydrobiopterin inhibits tumor angiogenesis in BALB/c-nu mice with hepatocellular carcinoma.

Authors - Youguo Dai

Life Sci

The expression of bitter taste receptors in mesenteric, cerebral and omental arteries.

Authors - Jing-Guo Chen
mouse

Int J Biol Sci

TIPE regulates VEGFR2 expression and promotes angiogenesis in colorectal cancer.

Authors - Mengya Zhong