|Positive WB detected in||BxPC-3 cells, Y79 cells, K-562 cells, L02 cells|
|Positive IP detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
20359-1-AP targets PER2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||goat, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||period homolog 2 (Drosophila)|
|Calculated molecular weight||137 kDa|
|Observed molecular weight||136 kDa|
|GenBank accession number||NM_022817|
|Gene ID (NCBI)||8864|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. PER2 is a negative element in the circadian transcriptional loop. PER2 influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. It negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. The antibody is specific to PER2. It can recognize isoform1 and isoform2 of PER2.
Pathophysiological significance of clock genes BMAL1 and PER2 as erythropoietin-controlling factors in acute blood hemorrhage.
Cell Death Dis
The circadian clock gene Bmal1 facilitates cisplatin-induced renal injury and hepatization.
Cell Death Dis
hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.
Int J Mol Sci
Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation.
Construction of recombinant pEGFP-N1-hPer2 plasmid and its expression in osteosarcoma cells.
J Dairy Sci
Ruminal epithelial cell proliferation and short-chain fatty acid transporters in vitro are associated with abundance of period circadian regulator 2 (PER2).