PER2 Polyclonal antibody

PER2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20359-1-AP

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)



Tested Applications

Positive WB detected inBxPC-3 cells, Y79 cells, K-562 cells, L02 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20359-1-AP targets PER2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name period homolog 2 (Drosophila)
Calculated Molecular Weight 137 kDa
Observed Molecular Weight 136 kDa
GenBank Accession NumberNM_022817
Gene Symbol PER2
Gene ID (NCBI) 8864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. PER2 is a negative element in the circadian transcriptional loop. PER2 influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. It negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. The antibody is specific to PER2. It can recognize isoform1 and isoform2 of PER2.

Protocols

Product Specific Protocols
WB protocol for PER2 antibody 20359-1-APDownload protocol
IP protocol for PER2 antibody 20359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Authors - Zandra E Walton
humanWB

Cell Metab

MYC Disrupts the Circadian Clock and Metabolism in Cancer Cells.

Authors - Brian J Altman
humanWB

Sci Adv

Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.

Authors - Yool Lee
humanWB

Nat Commun

Correspondence: Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα.

Authors - Brian J Altman
humanWB, IF, IP

Elife

KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.

Authors - Yool Lee
mouseWB

Elife

NRF2 regulates core and stabilizing circadian clock loops, coupling redox and timekeeping inMus musculus.

Authors - Ryan S Wible