PES1 Polyclonal antibody, PBS Only

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• PES1 Polyclonal antibody, PBS Only (13553-1-PBS)

• 

  WB analysis of COLO 320 using 13553-1-AP (same clone as 13553-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 13553-1-AP (same clone as 13553-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

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  IP experiment of COLO 320 using 13553-1-AP (same clone as 13553-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:300. This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13553-1-AP (same clone as 13553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13553-1-AP (same clone as 13553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13553-1-AP (same clone as 13553-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13553-1-AP (PES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13553-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PES1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13553-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

13553-1-PBS targets PES1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4342

  Product name: Recombinant human PES1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 238-588 aa of BC032489

  Sequence: LYQLLNLHYPPKLEGQAQAEAKAGEGTYALDSESCMEKLAALSASLARVVVPATEEEAEVDEFPTDGEMSAQEEDRRKELEAQEKHKKLFEGLKFFLNREVPREALAFIIRSFGGEVSWDKSLCIGATYDVTDSRITHQIVDRPGQQTSVIGRCYVQPQWVFDSVNARLLLPVAEYFSGVQLPPHLSPFVTEKEGDYVPPEKLKLLALQRGEDPGNLNESEEEEEEDDNNEGDGDEEGENEEEEEDAEAGSEKEEEARLAALEEQRMEGKKPRVMAGTLKLEDKQRLAQEEESEAKRLAIMMMKKREKYLYQKIMFGKRRKIREANKLAEKRKAHDEAVRSEKKAKKARPE

• Full Name: pescadillo homolog 1, containing BRCT domain (zebrafish)
• Calculated Molecular Weight: 588 aa, 68 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC032489
• Gene Symbol: PES1
• Gene ID (NCBI): 23481
• RRID: AB_2161967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00541
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.