PFKFB4 Polyclonal antibody, PBS Only

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• PFKFB4 Polyclonal antibody, PBS Only (29902-1-PBS)

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  WB analysis using 29902-1-AP (same clone as 29902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29902-1-AP (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29902-1-AP (same clone as 29902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29902-1-AP (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29902-1-AP (same clone as 29902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29902-1-AP (same clone as 29902-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 29902-1-AP (same clone as 29902-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using PFKFB4 antibody (29902-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

PFKFB4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29902-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4

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Product Information

29902-1-PBS targets PFKFB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30571

  Product name: Recombinant human PFKFB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 139-232 aa of NM_001317134

  Sequence: ATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVAD

• Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_001317134
• Gene Symbol: PFKFB4
• Gene ID (NCBI): 5210
• RRID: AB_2935486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16877
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), is a bi-function enzyme. On one hand, PFKFB4 synthesizes fructose 2,6-bisphosphate (F2,6-BP) to stimulate glycolysis, playing important roles in metabolic processes. On the other hand, PFKFB4 acts as a phosphatase to hydrolyze F2,6-BP into fructose-6-phosphate (F6P) and inorganic phosphate (Pi), functioning as a protein kinase to phosphorylate protein substrates. The dual function of PFKFB4 makes it involved in multiple biological processes such as cell cycle regulation, cell proliferation and transcriptional regulation(PMID: 33593309). PFKFB4 has been reported to be involved in a variety of cancers including human bladder cancer, gastric cancer, colon cancer, breast cancer and other malignant tumors(PMID: 21396842, PMID: 33732372, PMID: 31244553).