PHGDH Monoclonal antibody

PHGDH Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1E8B8

Reviews (1)

Cat no : 67591-1-Ig

Synonyms

EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH, 3 PGDH

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Validation Data Gallery

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  WB analysis using 67591-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67591-1-Ig (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 67591-1-Ig

  IP result of anti-PHGDH (IP:67591-1-Ig, 4ug; Detection:67591-1-Ig 1:3000) with HeLa cells lysate 980 ug.

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  IHC staining of human urothelial carcinoma using 67591-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67591-1-Ig (PHGDH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67591-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PHGDH antibody (67591-1-Ig, Clone: 1E8B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67591-1-Ig targets PHGDH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PHGDH fusion protein Ag6877
• Full Name: phosphoglycerate dehydrogenase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC000303
• Gene Symbol: PHGDH
• Gene ID (NCBI): 26227
• RRID: AB_2882799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).

Publications

Species	Application	Title
human	WB	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
mouse	WB	Int Immunopharmacol; Artesunate attenuates serum amyloid A-induced M1 macrophage differentiation through the promotion of PHGDH; Authors - Xinhui Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (11-30-2022)

Good antibody for WB. Strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse liver, mouse brain, and Hela cells.

PHGDH Antibody Western Blot validation (1:4000 dilution) in Mouse liver, mouse brain, and Hela cells. (Cat no:67591-1-Ig)