PHLPP Monoclonal antibody

PHLPP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat, pig and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E8E6

Cat no : 67640-1-Ig

Synonyms

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

67640-1-Ig targets PHLPP in WB, ELISA applications and shows reactivity with Human, mouse , rat, pig samples.

Tested Reactivity Human, mouse , rat, pig
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PHLPP fusion protein Ag18982
Full Name PH domain and leucine rich repeat protein phosphatase
Calculated Molecular Weight 1717 aa, 184 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC126277
Gene Symbol PHLPP
Gene ID (NCBI) 23239
RRIDAB_2882840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.

Protocols

Product Specific Protocols
WB protocol for PHLPP antibody 67640-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway.

Authors - Zong-Ze Zhang
pigWB

Pediatr Res

Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets

Authors - Agus Suryawan
humanWB

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu