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PIP4K2C Polyclonal antibody, PBS Only (17077-1-PBS)

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WB analysis using 17077-1-AP (same clone as 17077-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17077-1-PBS in a different storage buffer formulation.
WB analysis using 17077-1-AP (same clone as 17077-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17077-1-PBS in a different storage buffer formulation.
WB analysis using 17077-1-AP (same clone as 17077-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17077-1-PBS in a different storage buffer formulation.
WB analysis using 17077-1-AP (same clone as 17077-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17077-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 17077-1-AP (same clone as 17077-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17077-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 17077-1-PBS
HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17077-1-PBS
HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 17077-1-AP (same clone as 17077-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17077-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 17077-1-AP (same clone as 17077-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17077-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PIP4K2C Polyclonal antibody, PBS Only

PIP4K2C Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17077-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

17077-1-PBS targets PIP4K2C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10989 Product name: Recombinant human PIP4K2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-421 aa of BC028596 Sequence: MASSSVPPATVSAATAGPGPGFGFASKTKKKHFVQQKVKVFRAADPLVGVFLWGVAHSINELSQVPPPVMLLPDDFKASSKIKVNNHLFHRENLPSHFKFKEYCPQVFRNLRDRFGIDDQDYLVSLTRNPPSESEGSDGRFLISYDRTLVIKEVSSEDIADMHSNLSNYHQYIVKCHGNTLLPQFLGMYRVSVDNEDSYMLVMRNMFSHRLPVHRKYDLKGSLVSREASDKEKVKELPTLRDMDFLNKNQKVYIGEEEKKIFLEKLKRDVEFLVQLKIMDYSLLLGIHDIIRGSEPEEEAPVREDESEVDGDCSLTGPPALVGSYGTSPEGIGGYIHSHRPLGPGEFESFIDVYAIRSAEGAPQKEVYFMGLIDILTQYDAKKKAAHAAKTVKHGAGAEISTVHPEQYAKRFLDFITNIFA Predict reactive species
Full Name	phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Calculated Molecular Weight	421 aa, 47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	BC028596
Gene Symbol	PIP4K2C
Gene ID (NCBI)	79837
RRID	AB_2715526
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TBX8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PIP4K2C is involved in several processes, including 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; negative regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity; and positive regulation of autophagosome assembly. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism.