PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 26899-1-AP

Synonyms

PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13

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Validation Data Gallery

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  WB analysis using 26899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26899-1-AP (PKC zeta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 26899-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26899-1-AP (PKC Zeta antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 26899-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 26899-1-AP (PKC zeta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 26899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PKC Zeta antibody (26899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 26899-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Zeta antibody (26899-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HT-29 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

26899-1-AP targets PKC Zeta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKC Zeta fusion protein Ag25391
• Full Name: protein kinase C, zeta
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC014270
• Gene Symbol: PKC Zeta
• Gene ID (NCBI): 5590
• RRID: AB_2880675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.

Publications

Species	Application	Title
human	WB	PLoS Pathog; HRS plays an important role for TLR7 signaling to orchestrate inflammation and innate immunity upon EV71 infection.; Authors - Zhen Luo
mouse	WB	JCI Insight; Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.; Authors - Hongyi Zhou
	WB	Fish Shellfish Immunol; In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.; Authors - Zhong-Wen Chang
mouse	WB	J Cell Mol Med; Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis.; Authors - Qianxia Yin
human	WB	Cell Rep Med; Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling; Authors - Baoyu He ; KD Validated
human	IF	Dev Cell; Epithelial aPKC deficiency leads to stem cell loss preceding metaplasia in colorectal cancer initiation; Authors - Hiroto Kinoshita