CoraLite® Plus 488-conjugated PLCL2 Monoclonal antibody

PLCL2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C8A4

Cat no : CL488-67471

Synonyms

FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67471

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488-conjugated PLCL2 antibody (CL488-67471, Clone: 1C8A4 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67471 targets PLCL2 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLCL2 fusion protein Ag11461
• Full Name: phospholipase C-like 2
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 126 kDa
• GenBank Accession Number: BC036392
• Gene Symbol: PLCL2
• Gene ID (NCBI): 23228
• RRID: AB_2919481
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. (PMID: 24916648, 25880423)