CoraLite® Plus 647-conjugated PLOD2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PLOD2-Specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-21214

Synonyms

LH2, Lysyl hydroxylase 2, PLOD2, TLH

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Validation Data Gallery

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  FC experiment of HeLa using CL647-21214

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PLOD2-Specific (CL647-21214) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-21214 is PLOD2-Specific Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLOD2-Specific fusion protein Ag15614
• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Calculated Molecular Weight: 758 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC037169
• Gene Symbol: PLOD2
• Gene ID (NCBI): 5352
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.