PNKP Polyclonal antibody, PBS Only

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• PNKP Polyclonal antibody, PBS Only (13558-1-PBS)

• 

  WB analysis of Jurkat using 13558-1-AP (same clone as 13558-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13558-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13558-1-AP (same clone as 13558-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13558-1-AP (same clone as 13558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13558-1-AP (same clone as 13558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13558-1-PBS in a different storage buffer formulation.

PNKP Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 13558-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

13558-1-PBS targets PNKP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4421

  Product name: Recombinant human PNKP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 171-521 aa of BC033822

  Sequence: DLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

• Full Name: polynucleotide kinase 3'-phosphatase
• Calculated Molecular Weight: 521 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC033822
• Gene Symbol: PNKP
• Gene ID (NCBI): 11284
• RRID: AB_2877958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96T60
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.