CoraLite® Plus 647-conjugated PNPT1 Monoclonal antibody

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  IF Staining of A431 using CL647-68309

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 PNPT1 antibody (CL647-68309, Clone: 3F2A1 ) at dilution of 1:200, CL488-phalloidin (green).

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  IF Staining of HeLa using CL647-68309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 PNPT1 antibody (CL647-68309, Clone: 3F2A1 ) at dilution of 1:200, CL488-phalloidin (green).

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  FC experiment of HepG2 using CL647-68309

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 647 Pnpt1 Monoclonal Antibody (CL647-68309, Clone:3F2A1)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PNPT1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-68309

Clone No.3F2A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

3'-5' RNA exonuclease OLD35, 3F2A1, EC:2.7.7.8, old 35, OLD35

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Tested Applications

• Positive IF/ICC detected in: A431 cells, HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-68309 targets PNPT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PNPT1 fusion protein Ag6290
• Full Name: polyribonucleotide nucleotidyltransferase 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC053660
• Gene Symbol: PNPT1
• Gene ID (NCBI): 87178
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8TCS8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.