POLG2 Recombinant antibody

POLG2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85852-4-RR
Clone No.250198G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, H9C2 cells, mouse colon tissue, rat colon tissue, COLO 320 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85852-4-RR targets POLG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POLG2 fusion protein Ag1424 Predict reactive species
Full Name polymerase (DNA directed), gamma 2, accessory subunit
Calculated Molecular Weight 55 kDa
Observed Molecular Weight52-55 kDa
GenBank Accession NumberBC009194
Gene Symbol POLG2
Gene ID (NCBI) 11232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in cell lines with a single band of 52-55kd.

Protocols

Product Specific Protocols
WB protocol for POLG2 antibody 85852-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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