POLR3A Polyclonal antibody, PBS Only

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• POLR3A Polyclonal antibody, PBS Only (17530-1-PBS)

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  WB analysis using 17530-1-AP (same clone as 17530-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17530-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17530-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 17530-1-AP (same clone as 17530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17530-1-AP (POLR3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17530-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 17530-1-AP (same clone as 17530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17530-1-AP (POLR3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17530-1-PBS in a different storage buffer formulation.

POLR3A Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17530-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

17530-1-PBS targets POLR3A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11536

  Product name: Recombinant human POLR3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1041-1390 aa of BC041089

  Sequence: IGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEVFLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDTNEFHIPLVT

• Full Name: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• Calculated Molecular Weight: 1390 aa, 156 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC041089
• Gene Symbol: POLR3A
• Gene ID (NCBI): 11128
• RRID: AB_2167983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14802
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).