PPFIBP1 Polyclonal antibody

PPFIBP1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13961-1-AP

Synonyms

PTPRF-interacting protein-binding protein 1, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1, Liprin-beta-1, KIAA1230, hSGT2

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Validation Data Gallery

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  WB analysis of HepG2 using 13961-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13961-1-AP (PPFIBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13961-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13961-1-AP (PPFIBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13961-1-AP

  IP result of anti-PPFIBP1 (IP:13961-1-AP, 4ug; Detection:13961-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IF Staining of U2OS using 13961-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PPFIBP1 antibody (13961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 13961-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PPFIBP1 Polyclonal antibody (13961-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, DU 145 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13961-1-AP targets PPFIBP1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPFIBP1 fusion protein Ag4884
• Full Name: PTPRF interacting protein, binding protein 1 (liprin beta 1)
• Calculated Molecular Weight: 1011 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC050281
• Gene Symbol: PPFIBP1
• Gene ID (NCBI): 8496
• RRID: AB_2171631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Open Biol; The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions; Authors - Xingchen Li
human		J Inflamm Res; Development and Validation of Diagnostic Models for Transcriptomic Signature Genes for Multiple Tissues in Osteoarthritis; Authors - Qichang Gao