PPIG Polyclonal antibody, PBS Only

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• PPIG Polyclonal antibody, PBS Only (12985-1-PBS)

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  WB analysis using 12985-1-AP (same clone as 12985-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12985-1-AP (same clone as 12985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12985-1-AP (PPIG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12985-1-AP (same clone as 12985-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12985-1-AP (PPIG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12985-1-AP (same clone as 12985-1-PBS)

  Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12985-1-AP (same clone as 12985-1-PBS)

  Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

PPIG Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12985-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CARS Cyp, CARS-cyclophilin, CARS-Cyp, CASP10, Clk-associating RS-cyclophilin

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Product Information

12985-1-PBS targets PPIG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3622

  Product name: Recombinant human PPIG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-355 aa of BC001555

  Sequence: MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVVFGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSSSDSDSSSDSQSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPIPENRFLMRKSPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRVGDSFPRDLHNIAFVF

• Full Name: peptidylprolyl isomerase G (cyclophilin G)
• Calculated Molecular Weight: 739 aa, 87 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC001555
• Gene Symbol: PPIG
• Gene ID (NCBI): 9360
• RRID: AB_2169281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13427
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Peptidyl-prolyl cis-trans isomerase G (commonly abbreviated as PPIG) belongs to the cyclophilin subfamily of peptidyl-prolyl cis-trans isomerases (PPIases), which act as molecular chaperones to accelerate protein folding and regulate vital cellular processes.