PRICKLE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRICKLE1 Polyclonal Antibody for WB, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 22589-1-AP

Synonyms

PRICKLE1, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1, RILP, EPM1B

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Validation Data Gallery

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  WB analysis of mouse brain using 22589-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22589-1-AP (PRICKLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 22589-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 22589-1-AP (PRICKLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 22589-1-AP

  IP result of anti-PRICKLE1 (IP:22589-1-AP, 3ug; Detection:22589-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

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  IF Staining of mouse cerebellum using 22589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PRICKLE1 antibody (22589-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 22589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PRICKLE1 antibody (22589-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 22589-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PRICKLE1 (22589-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Neuro-2a cells
• Positive IP detected in: mouse brain tissue
• Positive IF-P detected in: mouse cerebellum tissue
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 4 publications below

Product Information

22589-1-AP targets PRICKLE1 in WB, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRICKLE1 fusion protein Ag18407
• Full Name: prickle homolog 1 (Drosophila)
• Calculated Molecular Weight: 831 aa, 94 kDa
• Observed Molecular Weight: 94 kDa, 55-60 kDa
• GenBank Accession Number: BC114939
• Gene Symbol: PRICKLE1
• Gene ID (NCBI): 144165
• RRID: AB_2879129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex.; Authors - Yue Ban ; KO Validated
chicken	WB	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
		Glia; The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.; Authors - Andrew A Jarjour
mouse	WB	Exp Neurol; Mutation of the murine Prickle1 (R104Q) causes phenotypes analogous to human symptoms of epilepsy and autism.; Authors - Yue Ban
human	WB	J Transl Med; PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia.; Authors - Duanfeng Jiang
	IF	bioRxiv; Differential Sensitivity of Midline Patterning to Mitosis during and after Primitive Streak Extension; Authors - Zhiling Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Murali (Verified Customer) (10-31-2022)

I will recommend this antibody for IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200

PRICKLE1 Antibody Immunofluorescence validation (1 to 200 dilution) in (Cat no:22589-1-AP)