PRIM1 Recombinant antibody

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  WB analysis using 86051-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86051-1-RR (PRIM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86051-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86051-1-RR against Human PRIM1 were performed. The affinity constant is 27.2 pM.

PRIM1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86051-1-RR

Clone No.250690B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DNA primase 49 kDa subunit, DNA primase small subunit, EC:2.7.7.102, p49

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, Sp2/0 cells, MIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86051-1-RR targets PRIM1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRIM1 fusion protein Ag1124
• Full Name: primase, DNA, polypeptide 1 (49kDa)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC005266
• Gene Symbol: PRIM1
• Gene ID (NCBI): 5557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49642
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subunit of this complex.