PRKCDBP Polyclonal antibody, PBS Only

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• PRKCDBP Polyclonal antibody, PBS Only (16250-1-PBS)

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  WB analysis of HeLa using 16250-1-AP (same clone as 16250-1-PBS)

  WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  WB analysis using 16250-1-AP (same clone as 16250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 16250-1-AP (same clone as 16250-1-PBS)

  IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 1120 ug. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 16250-1-AP (same clone as 16250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PRKCDBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16250-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein

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Product Information

16250-1-PBS targets PRKCDBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9209

  Product name: Recombinant human PRKCDBP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-261 aa of BC011585

  Sequence: MRESALEPGPVPEAPAGGPVHAVTVVTLLEKLASMLETLRERQGGLARRQGGLAGSVRRIQSGLGALSRSHDTTSNTLAQLLAKAERVSSHANAAQERAVRRAAQVQRLEANHGLLVARGKLHVLLFKEEGEVPASAFQKAPEPLGPADQSELGPEQLEAEVGESSDEEPVESRAQRLRRTGLQKVQSLRRALSGRKGPAAPPPTPVKPPRLGPGRSAEAQPEAQPALEPTLEPEPPQDTEEDPGRPGAAEEALLQMESVA

• Full Name: protein kinase C, delta binding protein
• Calculated Molecular Weight: 261 aa, 28 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC011585
• Gene Symbol: PRKCDBP
• Gene ID (NCBI): 112464
• RRID: AB_2171894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969G5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta