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PSMA3 Polyclonal antibody, PBS Only (11887-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of A549 using 11887-1-AP (same clone as 11887-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
WB analysis of human lung using 11887-1-AP (same clone as 11887-1-PBS)
human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 11887-1-AP (same clone as 11887-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
WB analysis using 11887-1-AP (same clone as 11887-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 11887-1-AP (same clone as 11887-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11887-1-AP (same clone as 11887-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

PSMA3 Polyclonal antibody, PBS Only

Name: PSMA3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11887-1-PBS

PSMA3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11887-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PSC8, Proteasome subunit alpha type-3, HC8

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

11887-1-PBS targets PSMA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2495 Product name: Recombinant human PSMA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC029402 Sequence: MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADMAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM Predict reactive species
Full Name	proteasome (prosome, macropain) subunit, alpha type, 3
Calculated Molecular Weight	255 aa, 28 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC029402
Gene Symbol	PSMA3
Gene ID (NCBI)	5684
RRID	AB_2171420
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P25788
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.