PSMA6 Polyclonal antibody

PSMA6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11573-1-AP

Synonyms

Proteasome subunit alpha type-6, PROS-27, PROS27, PROS 27, p27K

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Validation Data Gallery

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  WB analysis using 11573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11573-1-AP (PSMA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11573-1-AP

  IP result of anti-PSMA6 (IP:11573-1-AP, 4ug; Detection:11573-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

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  IHC staining of human lung cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse colon using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11573-1-AP

  Immunofluorescent analysis of HeLa cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 11573-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11573-1-AP

  Immunofluorescent analysis of HepG2 cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11573-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human PSMA6 (11573-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, Neuro-2a cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human testis tissue, mouse colon tissue, human thyroid cancer tissue, mouse testis tissue, rat small intestine tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells, HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

11573-1-AP targets PSMA6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, oyster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMA6 fusion protein Ag2154
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 6
• Calculated Molecular Weight: 246 aa, 27 kDa
• Observed Molecular Weight: 27-29 kDa
• GenBank Accession Number: BC023659
• Gene Symbol: PSMA6
• Gene ID (NCBI): 5687
• RRID: AB_2171742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.

Publications

Species	Application	Title
human	WB,IF	Small; Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis; Authors - Qian-Wei Luo
oyster	WB,IF	Proteomics; A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas.; Authors - Xiaohong Huang
	WB	Fish Shellfish Immunol; Mucous cell histopathology and label-free quantitative proteomic analysis of skin mucus in fat greenling (Hexagrammos otakii) infected with Vibrio harveyi; Authors - Xiaoyan Wei

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C