CoraLite® Plus 488-conjugated PSMA6 Monoclonal antibody

PSMA6 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D4B2

Cat no : CL488-67695

Synonyms

PROS-27, PROS27, PROS 27, p27K, IOTA

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Validation Data Gallery

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  IF Staining of human lung cancer using CL488-67695

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 PSMA6 antibody (CL488-67695, Clone: 1D4B2 ) at dilution of 1:200.

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  IF Staining of U2OS using CL488-67695

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 PSMA6 antibody (CL488-67695, Clone: 1D4B2 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human lung cancer tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67695 targets PSMA6 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMA6 fusion protein Ag21761
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 6
• Calculated Molecular Weight: 246 aa, 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC023659
• Gene Symbol: PSMA6
• Gene ID (NCBI): 5687
• RRID: AB_2919523
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.