PSMA8 Polyclonal antibody

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• PSMA8 Polyclonal antibody (14022-1-AP)

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• KD/KO Validated

PSMA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14022-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha4s, Proteasome alpha 4 subunit, Proteasome subunit alpha-type 7-like, Proteasome subunit alpha-type 8, PSMA7L

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  WB analysis using 14022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 14022-1-AP

  WB result of PSMA8 antibody (14022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 14022-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 14022-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14022-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSMA8 antibody 14022-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 14022-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA8 Polyclonal antibody (14022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, mouse thymus tissue, HeLa cells, rat thymus tissue
• Positive IHC detected in: human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14022-1-AP targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5138

  Product name: Recombinant human PSMA8 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-250 aa of BC042820

  Sequence: MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERTVRKICALDDHVCMAFAGLTADARVVINRARVECQSHKLTVEDPVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDDDGISRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTEDAIASDSEAIKLAIKALLEVVQSGGKNIELAIIRRNQPLKMFSAKEVELYVTEIEKEKEEAEKKKSKKSV

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 8
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC042820
• Gene Symbol: PSMA8
• Gene ID (NCBI): 143471
• RRID: AB_10896314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TAA3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PSMA8 Polyclonal antibody (Cat# 14022-1-AP) has 6 citations published in Nucleic Acids Research, Cell Death & Disease, Science China Life Sciences, Zoological Research, PLoS Genetics, and Nature Structural & Molecular Biology. The associated research fields include spermatogenesis, meiosis, proteasome biology, epigenetic reprogramming, and sperm nuclear architecture.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nucleic Acids Res; H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis.; Authors - Zhiming Li
mouse	WB	Cell Death Dis; Loss of full-length DAZL isoform disrupts PABPC1-dependent translational regulation and meiosis.; Authors - Xin Li
mouse	WB	Sci China Life Sci; Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice; Authors - Yiling Zhou
		Zool Res; Both 20S and 19S proteasome components are essential for meiosis in male mice; Authors - Ting-Ting Han
mouse	IF	PLoS Genet; The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.; Authors - Laura Gómez-H
		Nat Struct Mol Biol; Molecular architecture and spatial organization of proteasomes in the human sperm nucleus.; Authors - Piotr Kolata