PSMD4 Polyclonal antibody, PBS Only

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• PSMD4 Polyclonal antibody, PBS Only (14899-1-PBS)

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  WB analysis using 14899-1-AP (same clone as 14899-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14899-1-AP (same clone as 14899-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14899-1-AP (same clone as 14899-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 14899-1-AP (same clone as 14899-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14899-1-AP (same clone as 14899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14899-1-AP (same clone as 14899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14899-1-AP (same clone as 14899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14899-1-AP (same clone as 14899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14899-1-AP (same clone as 14899-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMD4 antibody (14899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 14899-1-AP (same clone as 14899-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PSMD4 (14899-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14899-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PSMD4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 14899-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

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Product Information

14899-1-PBS targets PSMD4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6691

  Product name: Recombinant human PSMD4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-377 aa of BC002365

  Sequence: MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002365
• Gene Symbol: PSMD4
• Gene ID (NCBI): 5710
• RRID: AB_2170627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55036
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.