PSPC1 Recombinant monoclonal antibody

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  IF Staining of HepG2 using 84657-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSPC1 antibody (84657-5-RR, Clone: 242158C1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84657-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSPC1 antibody (84657-5-RR, Clone: 242158C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PSPC1 Uni-rAb^® Recombinant Antibody for IF/ICC, ELISA

Cat No. 84657-5-RR

Clone No.242158C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

242158C1, PSP1, PSPC 1

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84657-5-RR targets PSPC1 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10136

  Product name: Recombinant human PSPC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC014184

  Sequence: MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLR

• Full Name: paraspeckle component 1
• Calculated Molecular Weight: 523 aa, 59 kDa
• GenBank Accession Number: BC014184
• Gene Symbol: PSPC1
• Gene ID (NCBI): 55269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8WXF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Paraspeckle component 1 (PSPC1) is a paranuclear spot protein, which is known as a paraspeckle biomarker and is a putative transcription factor that belongs to the Drosophila behavior/human splicing family with limited knowledge about its role in cancers. High PSPC1 expression in cancer tissues compared to corresponding benign tissues is associated with late tumor stages in patients with cancer and poor patient survival in breast, lung, and liver cancers.