Tested Applications
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-84657-5 targets PSPC1 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10136 Product name: Recombinant human PSPC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC014184 Sequence: MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLR Predict reactive species |
| Full Name | paraspeckle component 1 |
| Calculated Molecular Weight | 523 aa, 59 kDa |
| GenBank Accession Number | BC014184 |
| Gene Symbol | PSPC1 |
| Gene ID (NCBI) | 55269 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WXF1 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Paraspeckle component 1 (PSPC1) is a paranuclear spot protein, which is known as a paraspeckle biomarker and is a putative transcription factor that belongs to the Drosophila behavior/human splicing family with limited knowledge about its role in cancers. High PSPC1 expression in cancer tissues compared to corresponding benign tissues is associated with late tumor stages in patients with cancer and poor patient survival in breast, lung, and liver cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PSPC1 antibody CL488-84657-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
