PTCH1 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• PTCH1 Polyclonal antibody, PBS Only (17520-1-PBS)

• 

  WB analysis of HeLa using 17520-1-AP (same clone as 17520-1-PBS)

  WB result of PTCH1 antibody (17520-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTCH1 transfected HeLa cells. This data was developed using the same antibody clone with 17520-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17520-1-AP (same clone as 17520-1-PBS)

  Jurkat cells lysate was subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17520-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17520-1-AP (same clone as 17520-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17520-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 17520-1-AP (same clone as 17520-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17520-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PTCH1 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 17520-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

HPE7, NBCCS, Patched, patched homolog 1 (Drosophila), Patched1

---

Product Information

17520-1-PBS targets PTCH1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: patched homolog 1 (Drosophila)
• Calculated Molecular Weight: 161 kDa
• Observed Molecular Weight: 161 kDa
• GenBank Accession Number: NM_000264
• Gene Symbol: PTCH1
• Gene ID (NCBI): 5727
• RRID: AB_2176561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13635
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTCH1 (patched homolog 1) is a twelve-pass transmembrane protein that acts as a receptor of Hedgehog signaling pathway (PMID: 8906794). Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). In the absence of Hedgehog proteins (SHH, IHH and DHH in humans), PTCH1 represses the activity of Smoothened (SMO). Binding of Hedgehog proteins to PTCH1 inhibits the repression of SMO, resulting in the activation of downstream targets through the Gli transcriptional effectors (PMID: 23719536; 17139287). The gene of PTCH1 is considered to be a tumor suppressor gene. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly.