PTH Recombinant antibody, PBS Only (Capture)

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  WB analysis of Recombinant protein using 84960-4-RR (same clone as 84960-4-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 84960-4-RR (PTH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84960-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01717-3

  Sandwich ELISA standard curve of MP01717-3, Human PTH Recombinant Matched Antibody Pair - PBS only. 84960-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3191. 84960-3-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

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  Sample test of MP01717-3

  Plasma of six individual healthy human donors was measured. The PTH concentration of detected samples was determined to be 54.2 ng/mL with a range of 32.7-102.4 pg/mL.

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  Sample test of MP01717-3

  Serum of six individual healthy human donors was measured. The PTH concentration of detected samples was determined to be 44.3 pg/mL with a range of 28.9-54.3 pg/mL.

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  Affinity and Kinetic Characterization of 84960-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84960-4-RR against Human PTH were performed. The affinity constant is below 1 pM.

PTH Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84960-4-PBS

Clone No.242608F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

parathyroid hormone, 242608F8, Parathormone, Parathyrin

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Product Information

84960-4-PBS targets PTH as part of a matched antibody pair:

MP01717-3: 84960-4-PBS capture and 84960-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTH fusion protein Eg3191
• Full Name: parathyroid hormone
• Calculated Molecular Weight: 13kDa
• GenBank Accession Number: NM_000315.4
• Gene Symbol: PTH
• Gene ID (NCBI): 5741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01270
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Parathyroid hormone (PTH) is a crucial hormone secreted by the parathyroid glands. The main function of PTH is to regulate calcium and phosphorus metabolism in vertebrates. It increases blood calcium levels and decreases blood phosphorus levels. PTH also plays a role in maintaining bone health and is involved in the regulation of vitamin D metabolism.